Fig. 5
Treatment of 3D cocultures with PIC-NP-Tal. Spheroid cocultures of OVCAR8-DsRed2 and NCI/ADR-RES-EGFP cells were treated with PIC-NP-Tal or relevant controls on day 4. Light-activation was performed at 0 (dark control), 20, or 50 J/cm2. Viability analysis using the CellTiter-Glo® Cell Viability Assay was performed, where luminescence values were normalized to the no treatment (NT) 0 J/cm2 control (a). Normalized luminescence is further analyzed within light doses for 0 (b), 20 (c), and 50 J/cm2 (d). Fluorescence-based viability of each cell line, normalized to untreated spheroids, is shown at each light dose for NP-Tal (e), BPD (f), PIC (g), BPD + NP-Tal (h), PIC + NP-Tal (i), and PIC-NP-Tal (j). * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001