Fig. 1

Schematic and characterization of talazoparib-loaded nanoparticles. NP-Tal is prepared by co-dissolution of polymers and talazoparib in acetone, which is added into water containing 0.1% Pluronic F-68 surfactant. The mixture is sonicated, and solvent is evaporated under constant spinning for 4–6 h, then nanoparticles are concentrated using 30 kDa MWCO centrifugal filter (a). Representative TEM images are shown (b). Scale bar: 250 nm. During optimization procedures, initial talazoparib (mg) was varied (c-g) and initial PLGA-PEG-COOH (mg) was varied (h–l). (m-q) Next PLGA-PEG-DBCO was mixed in with PLGA-PEG-COOH at varying amounts from 0–100% where total polymer mass remained fixed at 42.8 mg. The characterized parameters include loading capacity (%), encapsulation efficiency (%), size (nm), polydispersity index, and zeta potential. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001