Fig. 8

Impact of BBR on intestinal barrier function and bacterial translocation in Mdr2^−/− mice. a Serum levels of FITC-Dextran levels, a marker of intestinal permeability. b-c Colony-forming units (CFUs) of bacteria isolated from MLNs (mesenteric lymph node) and blood, respectively. d Representative images of H&E staining of small intestine sections (scale bar, 50 µm for 20 × and 20 µm for 40 × magnification). e Representative images of Alcian blue staining of small intestine sections (scale bar, 50 µm for 20 × and 20 µm for 40 × magnification). f Representative images of ZO-1 immunofluorescence (IF) staining of small intestine sections (scale bar, 20 µm for 40 × magnification). Data are expressed as the mean ± SEM. Statistical significance relative to Control: *p < 0.05, **p < 0.01, ***p < 0.001 (n = 9–12)