Fig. 6
Effect of BBR on bile acid metabolism in Mdr2−/− mice. a–c, f Relative mRNA levels of key genes involved in bile acid metabolism in the liver, including Cyp7a1, Shp (Nr0b2), Fxr(Nr1h4), Cyp27a1, Cyp7b1, Asbt, Nr4a1, Abcg5, and Ntcp. The mRNA levels were determined by qRT–PCR and normalized with HPRT1 as an internal control. d Representative immunoblot images of FXR, SHP, CYP7A1 and β-Actin are shown. e The relative protein levels of FXR, SHP, and CYP7A1 were calculated using β-actin as a loading control. Data are expressed as the mean ± SEM. Statistical significance relative to Control: *p < 0.05, **p < 0.01, ***p < 0.001 (n = 9–12)