Fig. 4

CYP19A1 is the potential target of METTL3-mediated translation regulation. (A-B) GO-KEGG analysis and GSEA of genes with decreased translation efficiency after METTL3 knockout indicated the association between METTL3 and the steroid signalling pathway. (C) Genes downregulated in translation efficiency after METTL3 depletion were crossed with genes involved in steroid biosynthetic processes and the cytochrome P450 enzyme system to obtain CYP19A1. (D-E) IHC staining analysis of patient tissue microarray showed that compared to adjacent tissues, CYP19A1 protein was highly expressed in both the primary lesions and metastatic lymph nodes of NSCLC. (F) The results of IHC analysis indicated that CYP19A1 expression was positively correlated with METTL3 expression in NSCLC. (G) Survival analysis of the tissue microarray indicated that high expression of CYP19A1 suggests poor prognosis of NSCLC patients. (H) Western blotting analysis validated that the CYP19A1 protein is highly expressed in NSCLC tissues compared with paired normal lung tissues. (I) Data from the TCGA database indicated that CYP19A1 mRNA expression in NSCLC is lower than that in normal lung tissue. (J) RT‒qPCR indicated that CYP19A1 mRNA expression in NSCLC is not higher than that in paired normal lung tissue