Fig. 2

METTL3 promotes migration, invasion and invadopodia formation in NSCLC cells. (A) Scanning electron microscopy showed that overexpression of METTL3 in A549 and H1975 cells resulted in increased and elongated pseudopodia, while knockout of METTL3 led to decreased and shortened pseudopodia in the cells. (B-C) In the fluorescence matrix degradation experiment, the depth and extent of extracellular matrix degradation were significantly reduced when METTL3 was knocked out in A549 cells using CRISPR‒Cas9 technology. Furthermore, the depth and extent of extracellular matrix degradation were reduced in H1975 cells after METTL3 knockdown. (D-E) The wound healing experiment after METTL3 knockout demonstrated a significant decrease in cell migration ability in A549 cells. After transfection with siRNA targeting METTL3, there is a significant decrease in the migration ability of H1975 cells. (F-G) Transwell invasion experiments after METTL3 knockout showed a significant decrease in cell invasion ability of A549 cells. After transfection with siRNA targeting METTL3, there is a significant decrease in the invasion ability of H1975 cells