Fig. 6

Rasal1 increases tubulin detyrosination. A EGFP-control, Rasal1-OE, and Rasal1-shRNA transduced hippocampal neurons stained for tyrosinated tubulin (red) and detyrosinated tubulin (green) at DIV6. Scale bar: 20 μm unless stated otherwise. B, C Quantification of fluorescence intensity measured in the mid region of the longest neurite of detyrosinated (B) and tyrosinated (C) tubulin as a proportion of total tubulin. n = 50 for each group, pooled from 5 repeated experiments, mean plus SEM are plotted, *** = p < 0.001, unpaired student t-test, two tailed