Fig. 5

Rasal1 inhibits dendrite length and branching. A Representative confocal images of EGFP-control, Rasal1-OE, and Rasal1-shRNA transduced mouse hippocampal neurons stained for the dendritic marker MAP2 (red) and axonal marker Tau1 (green) (left). Tracings of dendrites (middle) and axonal neurites (right) were done using SynD. Scale bar: 20 µm. B Total axon and dendrite length (µm) of EGFP-control, Rasal1-OE, and Rasal-shRNA expressing neurons. C Number of primary and secondary dendritic branches in neurons expressing EGFP-control, Rasal1-OE, and Rasal1-shRNA. Data information: In B, C Mean ± SEM are plotted, *p < 0.05, **p < 0.01, ***p < 0.001, unpaired student t-test, two tailed. n = 60 for each group, pooled from 3 repeated experiments