Fig. 3

Rasal1 binds to CaMKII, PKC, α- and β-tubulin. Confirmation of suspected Rasal1 binding partners using pull-down assay (n = 3) with GST tagged Rasal1 fragments; N-terminus (GST-n), middle fragment (GST-m) or C-terminal fragment (GST-c) validated by Co-IP. A Western blot of pull-down assay (left) and Co-IP (right) of GST-tagged Rasal1 fragments stained for CaMKII in hippocampal and whole brain lysate. B Western blot of pull-down assay (left) and Co-IP (right) of GST-tagged Rasal1 fragments stained for PKC in hippocampal and whole brain lysate. C Western blot of pull-down assay (left) and Co-IP (right) of GST-tagged Rasal1 fragments stained for β-tubulin in hippocampal and whole brain lysate