Fig. 7
From: Epigenetic reshaping through damage: promoting cell fate transition by BrdU and IdU incorporation
BrdU and IdU have functions in various reprogramming systems. A Images of GFP + colonies taken by fluorescence microscope in situ. Scale bar 5 mm. B Number of Oct4-GFP colonies generated under indicated conditions. n = 3, ∗P < 0.05, ∗∗∗∗P < 0.0001. C FSC analysis of Oct4-GFP colonies generated under different treatment conditions. D Heatmap of RNA-seq for KS at D4, 8, 12 and 16 with or without BrdU or IdU. E Morphological changes during induction of KS treated with ATM inhibitor and corresponding statistical analysis. Scale bar 50 \(\mu\)m. n = 3, ∗∗∗P < 0.001. F GATA4 Cut & tag analysis and ATAC-seq analysis for CIP at D4, 8, 12 and 16 under different treatment conditions. G Morphological changes during induction of KS when overexpressing Gata4 in the system. Scale bar 50 \(\mu\)m