Fig. 4
From: Epigenetic reshaping through damage: promoting cell fate transition by BrdU and IdU incorporation
Mechanism of DDR in promoting somatic cell reprogramming. A A model for DNA damage repair (DDR) mechanism during BrdU/IdU incorporation. B Morphological changes during induction of CiPSCs treated with or without ATM inhibitor (KU-55933). Scale bar 100 \(\mu\)m. C Number of Oct4-GFP + CiPSC colonies generated under different treatment conditions. n = 3, ∗∗P < 0.01. D Representative Gata4, Sox17 and Sall4 peaks from H3K27ac Cut & tag aligned with H3K9ac Cut & tag signals for CIP at D20 with or without BrdIr IdU. E Morphological changes during induction of CiPSCs treated with different concentration of VPA. Scale bar 100 \(\mu\)m. F Number of Oct4-GFP + CiPSC colonies generated under different treatment conditions. n = 3, ∗∗∗∗P < 0.0001. G GATA4 Cut & tag analysis and ATAC-seq analysis for CIP at D20 and D26 under different treatment conditions. H Representative Oct4, Sall4 and Aqp8 peaks from ATAC-seq aligned with H3K27ac Cut & tag and GATA4 Cut & tag for CIP at D20 with or without BrdU or IdU