Fig. 3
From: Epigenetic reshaping through damage: promoting cell fate transition by BrdU and IdU incorporation
Incorporation of BrdU/IdU leads to DNA damage repair. A Venn diagrams for overlapping genes in the upregulated groups between BrdU, IdU, and BrdU + IdU versus the control. B Enriched GO functions in upregulated groups. C Heatmap of DNA-repair genes related RNA-seq for CIP at D8, 14, 20 and 26 with or without BrdU or IdU. D Alkaline comet assays of samples with or without BrdU or IdU. Scale bar 50 \(\mu\)m. E Statistical analysis of olive tailmomer from D. F Average Olive Tail Moment from E. G Immunofluorescent analysis to observe the spatial colocalization of BrdU (in red) and γ-H2AX (in green). Scale bar 40 \(\mu\)m. H Representative peaks from ATAC-seq aligned with γ-H2AX Cut & tag signals for Gata4 and Sox17 with or without BrdU or IdU