Fig. 1
From: Epigenetic reshaping through damage: promoting cell fate transition by BrdU and IdU incorporation
BrdU and IdU: Essential in overcoming two barriers during chemical induction of pluripotency. A Schematic diagram of the induction of CiPSCs from MEFs. B Morphological changes at two distinct barriers during induction of CiPSCs. Scale bar 100 \(\mu\)m. C Number of Oct4-GFP + CiPSC colonies generated under indicated conditions. n = 3, ∗P < 0.05, ∗∗∗∗P < 0.0001. D Morphological changes at distinct time points during induction of CiPSCs treated with BrdU or IdU. Scale bar 100 \(\mu\)m. E Number of Oct4-GFP + CiPSC colonies generated under different treatment conditions. I,IdU; B,BrdU; I + B:IdU + BrdU, n = 3, ∗P < 0.05, ∗∗∗P < 0.001 and ∗∗∗∗P < 0.0001