Table 3 Comparison of human primary cells and cell lines
From: Precision nephrotoxicity testing using 3D in vitro models
| Â | Cell lines | Human primary cells | iPSC-derived cells |
|---|---|---|---|
| Â | HK-2, RPTEC/TERT1, LLC-PK1 | Proximal tubular epithelial cells, Mixed renal tubule cells, Podocytes | All renal cell types |
Advantages | • Reproducibility • Homogeneity • Availability | • High physiological relevance • Greater genetic diversity • Reduced culture artifacts • Improved translational potential | • Patient-specific models • Recapitulation of renal development • Cellular diversity • High-throughput screening |
Disadvantages | • Lack of physiological relevance • Limited diversity • Genetic drift • Cell culture artifacts | • Limited availability • Limited lifespan • Heterogeneity • High cost | • Immaturity • Variability • Complexity |