Table 1 Primary antibodies used
Antigen | Target | Host | Dilution | Supplier |
|---|---|---|---|---|
α-SMA | Dedifferentiation | Mouse | 1:1000 | Abcam |
β-III-Tubulin | RGCs | Rabbit | 1:2000 | Promega |
β-Catenin | Dedifferentiation | Rabbit | 1:2000 | Abcam |
CD-133 | Dedifferentiation | Rabbit | 1:200 | Abcam |
Nestin | Dedifferentiation | Rabbit | 1:500 | Sigma |
OCT4 | Dedifferentiation | Rabbit | 1:200 | Abcam |
p75NTR | Müller glia | Rabbit | 1:2000 | Abcam |
Piezo1 | Mechanosensor | Rabbit | 1:100 | Life Technologies |
Trpv4 | Mechanosensor | Rabbit | 1:500 | Life Technologies |
Vimentin | Müller glia | Mouse | 1:10,000 | Dako |