Fig. 7

The functional analysis of the proteins in the CM derived from central and peripheral MG under control conditions and when exposed to HP, and a STRING analysis for some selected proteins. Graphical representation of the principal functions of the proteins more represented in the CM from central (A) and peripheral (B) pig MG cultures in control conditions and when exposed to HP. C STRING analysis of three proteins that were more strongly expressed in the peripheral CM under HP conditions. When considering all proteins obtained in the STRING analysis, circles surround the proteins identified in the proteomic analysis, with red circles around proteins that were significantly more expressed in the peripheral CM, and black circles represent those present in peripheral CM with at least a twofold increase relative to the control peripheral MG CM. The three proteins represent some of the principal functions of the proteins found in the peripheral HP CM: TXN mediates the response to reactive oxygen species, ACTN4 induces apoptosis, and PSMA4 is involved in inflammation-related pathways and networks