Fig. 6

Immunolabelling of the pressure receptors PIEZO1 and TRPV4 in purified cultures of MG isolated from the central or peripheral pig retina under control conditions or HP. Images of cultured MG isolated from the central (A, B, E, F) and peripheral (C, D, G, H) retina in control conditions (A, E, C, G) and in response to EH (B, F, D, H). The cells were labelled with antibodies against PIEZO1 (A–D, red), TRPV4 (E–H, red) and vimentin (green). Nuclei were stained with DAPI (blue). Note the enhanced immunoreactivity of both pressure receptors in response to HP. TRPV4 is also expressed in the control peripheral MG. Scale bar: 50 µm