Fig. 4

Effect of HP on the survival of central and peripheral pig RGCs and MG in co-cultures. Images of co-cultured pig MG and RGCs from A the center and B the periphery of the retina under control condition, and from C the center and D the periphery of the retina after 72 h of HP. The cells were labelled with antibodies against β-III-Tubulin (red) and vimentin (green). Nuclei are stained with DAPI (blue). E, F Number of central and peripheral RGCs (E) or MG (F) in the co-cultures under control conditions and HP: *p-value < 0.05; Scale bar: 50 µm