Fig. 2

Immunolabelling of vimentin, CD133, OCT4, nestin, α-SMA, β-catenin and β-III Tubulin in pure cultures of MG isolated from the central and peripheral pig retina. Images of cultured MG isolated from the center (A, C, E, G, I) or periphery (B, D, F, H, J) of the retina. The cells were labelled with antibodies against CD133 (A, B, red), OCT4 (C, D, red), nestin (E, F, red), β-catenin (G, H, red), β-III Tubulin (I, J, red), α-SMA (E, F, green), or vimentin (A–J, green). Nuclei were stained with DAPI (blue). Note the increase in the expression of all stem cell-like markers and β-III-Tubulin in the peripheral MG. Scale bar: 50 µm