Fig. 1

A The survival of central or peripheral pig RGCs in purified cultures. Images of purified RGC cultures from the central and the peripheral retina. The same number of RGCs were seeded in both cases. Histogram of the number of RGCs in the cultures from each retinal region. B Effect of MG from the central and peripheral retina on the survival of RGCs from the central and peripheral retina, respectively, in co-cultures. Images of co-cultures of pig RGCs and MG from the central and peripheral retina. The MG and RGC in the co-cultures were both isolated from the same area of the retina (center and periphery). Number of RGCs in co-cultures from the center and periphery of the retina and number of MG in co-cultures from the center and periphery of the retina were represented. C The survival of rat RGCs seeded on confluent pig MG isolated from the central and peripheral retina. Images of rat RGCs seeded on pig MG from the central and peripheral retina. The same initial number of rat RGC were seeded on pig MG cultures. The number of rat RGCs present on pig MG cultures from the central or peripheral retina. D Survival and neuritogenesis of rat RGCs when maintained in CM secreted by MG from the central and peripheral retina. Images of rat RGCs maintained with conditioned medium (CM) from central and peripheral pig MG. The same number of RGCs were seeded in all conditions. Number of surviving rat RGCs maintained with CM secreted by MG from the central or peripheral pig retina was represented. To analyze neuritogenesis, the RGCs were classified as RGCs without neurites (blue), RGCs with the longest neurite < 50 μm (orange), RGCs with the longest neurite between 50 and 200 μm (grey), and those with neurites longer than 200 μm (yellow). The number of RGCs in each category is shown for those maintained in the presence of both types of CM. The RGCs were labelled with antibodies against β-III-Tubulin (red) and the MG with vimentin (green). Nuclei were stained with DAPI (blue): *p-value < 0.05. Scale bar: 50 µm