Fig. 7
Increased autophagy/mitophagy in Fkbp51 KO livers. A, C Immunoblots depict mitochondrial and total p-DRP1, DRP1, p62, PINK1, Parkin, and respective loading controls in CCl4-treated liver. B, D Densitometry ratios of target proteins relative to loading control in the mitochondrial and total fractions from A and C, respectively. E–G Immunoblots depict mitochondrial and total LC3B and respective loading controls in livers. Densitometry revealed higher LC3B II/I ratios in KO of mitochondria fraction and total protein following CCl4 treatment. Graphs represent mean values ± SEM from 6 mice for each group. p values were determined by student’s unpaired t-test for (B, D) or two-way ANOVA for (F, G) with the statistical significance labeled as follows: *as p < 0.05, **as p < 0.01, *** as p < 0.001 and **** as p < 0.0001. Key: WT, wild type; KO, Fkbp51 KO; Mito, mitochondria