Fig. 4

Transcriptome analysis of WT and the NR2F2-KO COV434 cells. A Volcano plot representing downregulated (log₂FC < − 1) and upregulated (log₂FC > 1) differentially expressed genes (DEGs) between NR2F2-KO and WT COV434 cells. Absolute theta > 2 was used as a cutoff. B Heatmap representing gene expression levels of cell markers associated with early gonadal somatic cells, extragonadal mesonephros, and endothelial cells in the RNA-seq of WT and NR2F2-KO COV434 cells. The values of fragments per million mapped fragments (FPM) are expressed relative to the WT mean. C Gene ontology analysis of biological processes for downregulated and upregulated DEGs (NR2F2-KO versus WT COV434 cells). D RT-qPCR assay comparing relative mRNA expression of transcripts related to early gonadal somatic cells (LHX9 and SOX4), testis (SOX9 and PDGFB), and ovary development (WNT4, CTNNB1, and FST) between WT and NR2F2-KO COV434 cell clones. S8 was used as a reference gene. Values are mean ± SEM (n = 3–4). Student’s t-test with Welch’s correction, *p < 0.05, **p < 0.01. Source data for this figure are available in Additional file 2