Fig. 6
Communication between beige adipocytes and MIIP downregulated CRC cells promotes tumor growth in vivo. a–c HCT116-MIIP+/− or WT cells (1 × 106 cells per mouse) were co-injected unilaterally with primary adipocytes (2.5 × 105 cells per mouse) into the back of nude mice (n = 5). Tumor growth was monitored every 3 days a. The tumors were removed (b, scale bar: 1 cm) and weighed (c) at the end of the experiment. d–e Representative images (d) and statistical analysis (e) of UCP1, CD36, FABP4, and Ki67 proteins by IHC staining and representative images of H&E staining in xenograft tumors derived from (B) (n = 5, scale bar: 50 μm, red). f Representative images of IHC staining of CD36 and Ki67 proteins and H and E staining in xenograft tumors derived from mice injected with HCT116 (MIIP+/− or WT) alone (n = 5, scale bar: 200 μm). g Body weights of mice monitored in (A) on day 22 (n = 5). h Representative images and statistical analysis of endogenous CD36 and FABP4 in clinically defined human colorectal cancer samples by IHC staining (Scale bar: 100 μm, red). Adipo: mature adipocytes. All data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001