Fig. 4
Miip downregulated cancer cells aggravate the browning of tumor-adjacent adipocytes in vivo. a–b Stable Miip-knockdown or scramble CT26.WT cells carring LUC (2 × 105 cells per mouse) were co-injected unilaterally with 3T3-L1 cells (5 × 104 cells per mouse) into the back of BALB/c mice. Tumor growth was monitored every 3 days a, and the tumors were removed (b, scale bar: 1 cm) at the end of the experiment (n = 5). c Representative bioluminescence images of tumor growth in mice described in a, b at day 21 (n = 5). d The tumors described in b were weighed at the end of the experiment. e Immunoblots of UCP1 and PGC-1α proteins in allograft tumors derived from b. f Representative images and statistical analysis of UCP1 and PGC-1α proteins in allograft tumors derived from b by IHC staining (n = 5, scale bar: 100 μm). All data are presented as mean ± SD. *P < 0.05, ***P < 0.001