Fig. 1

Autophagy is enhanced during the transition from telogen to anagen. A Representative immunofluorescence images of dorsal hair follicles stained with anti-LC3B antibody(red) and anti-K15 antibody(green) in early telogen, middle/late telogen, telogen-anagen transition and anagen. B Representative immunofluorescence images of dorsal hair follicles stained with anti-P62 antibody(green) and anti-K15 antibody(red) in early telogen, middle/late telogen, telogen-anagen transition and anagen. C Quantification of LC3B-fluorescent dots/cell in K15 + HFSCs region of dorsal hair follicles(left); Quantification of P62-fluorescent signal in K15 + HFSCs region of dorsal hair follicles(right). The data represent the means ± S.E.M from at least three independent experiments. *P < 0.05, **P < 0.01, ****P < 0.0001, determined by Student’s t-test, the other stages of hair follicle cycle versus telogen-anagen