Fig. 6From: mTOR hypoactivity leads to trophectoderm cell failure by enhancing lysosomal activation and disrupting the cytoskeleton in preimplantation embryoCombined analysis of DNA methylation pattern and transcriptional expression of blastocysts derived from mTOR inhibitor treatment during 8-cell to blastocyst development. A The morphology of blastocysts from the control (Con) and rapalink-1 (RPL) groups that were used for genome-wide bisulfite sequencing. B Heatmap showing the level of DNA methylation of the CpG site in each group of blastocysts. C The levels of genome-wide DNA methylation in RPL and Con are shown (n = 3). D The number and proportion of hypermethylated and hypomethylated differentially methylated regions (DMRs) in RPL vs Con. E Venn diagram of DMRs-associated genes (DMGs) and differentially expressed genes (DEGs) detected by pair-wise comparison. F Bar chart illustrating the number and proportion of hypermethylated-downregulated genes (Hyper-down), hypermethylated-upregulated genes (Hyper-up), hypomethylated-downregulated genes (Hypo-down) and hypomethylated-upregulated genes (Hypo-up) from overlapped genes in DMGs and DEGs. G Bar chart illustrating the enriched top GO terms associated with the overlapped genes of hyper-down. H Bar chart illustrating the enriched top GO terms associated with the overlapped genes of hyper-up. I Bar chart illustrating the top 10 enriched KEGG terms or canonical pathways in overlapped genes of hyper-down. J Bar chart illustrating the top 10 enriched KEGG terms or canonical pathways in overlapped genes of hyper-upBack to article page