Fig. 5From: mTOR hypoactivity leads to trophectoderm cell failure by enhancing lysosomal activation and disrupting the cytoskeleton in preimplantation embryoTranscription profiles of blastocysts and key gene terms enriched in mTOR inhibitor treatment during 8-cell to blastocyst development. A Volcano plot displaying the DEGs in RPL blastocysts. B Bar chart illustrating the enriched GO terms associated with the significantly downregulated transcripts in RPL blastocysts identified by RNA-Seq. C Bar chart illustrating the enriched GO terms associated with the significantly upregulated transcripts in RPL blastocysts. D GSEA plots showing trophectodermal cell differentiation enriched in RPL-blastocyst (left) and Con-blastocyst (right). NES, normalized enrichment score; FDR, false discovery rate. E Heatmap illustrating the relative expression level (row-scaling) of the genes associated with trophectodermal cell differentiation between RPL and Control blastocyst. F Bar chart illustrating the top ten enriched KEGG terms or canonical pathways in RPL blastocyst. G GSEA profiles showing the most significant enrichment of gene sets associated with lysosomes in RPL blastocysts (RPL vs Con). H The heatmap shows the expression pattern of lysosome-related genes in RPL and Con blastocysts. I Representative immunofluorescence images of lamp1 (lysosomal associated membrane protein 1) and LC3β (microtubule-associated protein 1 light chain 3β) in RPL and Con embryos. Bar, 50 μm. J The normalized intensity of lamp1 between Con and RPL group embryos (n = 10). K The normalized intensity of LC3β between Con and RPL group embryos (n = 10)Back to article page