Fig. 1From: mTOR hypoactivity leads to trophectoderm cell failure by enhancing lysosomal activation and disrupting the cytoskeleton in preimplantation embryoDevelopmental phenotypes of embryos from pregnancy mouse that were inhibition of mTOR signaling during preimplantation development. A Schematic illustration of the experimental setup of mouse treatment, embryo recovery and detection. B Immunostaining showing the levels of pS6 and p-AKT after 24 h of treatment with mTOR inhibitors at the 1-cell stage. Scale bars, 20 μm. C Representative bright-field images of embryos recovered from corn oil and RPL treated mice at 3.5 dpc. Scale bars, 100 μm. D The percentage of blastocysts was calculated at 3.5 dpc (n = 3). E Assessment of the implantation potential of blastocysts derived from corn oil and RPL treated mice (n = 12). F HE stained sections of paraffin-embedded embryos at 5.5 dpc. em, embryo. de, decidua. G Calculation of the number of fetuses recovered from the uteri at 13.5 dpc (n = 11). H Representative images showing conceptuses at 13.5 dpc and quantification of embryo crown-rump length (n = 43 for Corn oil and 64 for RPL). Production of offspring (I) (n = 10 for Corn oil and 12 for RPL) and the body weights (J) of offspring (n = 127 for Corn oil and 131 for RPL) from corn oil and RPL treated embryos. Error bars are mean ± SEM. ** p < 0.01, * p < 0.05, n.s., not significant (p > 0.05). RPL Rapalink-1, dpc days postcoitus, HE Hematoxylin and eosinBack to article page