Fig. 6

Characterization of p38α kinase activity on β-catenin. A In vitro kinase assay showing β-catenin phosphorylation by p38α in the absence or presence of ralimetinib at the indicated concentrations. Statistical analysis was performed using Student’s t-test: *P < 0.05 vs. active p38α; ^ΔP < 0.05 vs. active p38α + β-catenin. B MS/MS spectrum of the double-charged precursor ion of peptide A96AMFPETLDEGMQIPS111T112QFDAAHPTNVQR124. C In vitro kinase assay showing phosphorylation of β-catenin-WT, β-catenin-S111A, and β-catenin-T112A by p38α. Results are representative of at least three independent experiments