Fig. 4From: Uncoupling p38α nuclear and cytoplasmic functions and identification of two p38α phosphorylation sites on β-catenin: implications for the Wnt signaling pathway in CRC modelsImmunofluorescence analysis of p38α and β-catenin cellular localization. Immunofluorescence analysis of p38α and β-catenin cellular localization in HT-29 CRC cells under serum starvation (24 h) and upon activation of the Wnt pathway by serum supplementation or addition of Wnt3a (50 ng/mL) and the GSK3β inhibitor TWS-119 (10 μM) for 4 h. Subsequently, cells were treated or not with the p38α inhibitor ralimetinib (10 μM) for 24 h. Results are representative of at least three independent experimentsBack to article page