Fig. 2

Effect of p38α inhibition in a CRC mouse model. A Mice treatment scheme. APC^Min/+ mice were administered with AOM (14 mg/kg body weight) once a week for 5 weeks; one month later, they were subjected to daily intraperitoneal injections of the p38α inhibitor SB202190 (0.05 μmol/kg body weight) or DMSO for 14 days and then sacrificed. B Graph showing the reduction in the number of colon tumors in animals treated with SB202190. Tumors were stained with methylene blue, counted, and measured. Statistical analysis was performed using Student’s t-test: *P < 0.05 vs. DMSO. C Immunohistochemical analysis of p38α and β-catenin cytoplasmic and nuclear staining in C57BL/6 mice and AOM-treated APC^Min/+ mice injected with the p38α inhibitor SB202190 or DMSO. Original magnification: 100 × and 200 × . p38αi = p38α inhibitor. Results are representative of at least three independent experiments