Fig. 6

The inhibition of exosome biogenesis modulates the expression of migratory/invasive related genes in single-cell-derived clones. A Scheme representing the hypothetical mode of action of GW4869. Single-cell-derived clones in co-cultures were treated with the vehicle control (a) or with the GW4869 (b) for 96 h. B, C The graph-bar shows the fold change in mRNA expression between a clone grown in mono and co-culture conditions. The mRNA levels were analysed by Q-PCR for the indicated and selected set of genes involved in migration and invasion phenotypes and for the clones 1C5 and 2B4 (B) derived from OPBG-DIPG002 and the clones 5E2 and 1D3 derived from OPBG-GBM002 (C). D, E Graph-bars represent the fold change in mRNA expression of the indicated genes, analysed by Q-PCR between cells treated and not treated with 10 μM of GW4869 for the OPBG-DIPG002 derived clones 1C5 (D) and 2B4 (E) grown for 96 h in mono- (dash-bars) or in co-culture (plain coloured-bar) conditions. F, G Same as D and E but with the clones 5E2 (F) and 1D3 (G) derived from OPBG-GBM002 and treated with 20 μM of GW4869. For the gene see Table 1. Data are mean ± SD, n = 3. (****) p < 0.0001; (***) p < 0.001; (**) p < 0.01; (*) p < 0.05