Fig. 5

GW4869 treatment affects the migration capability of the single-cell-derived clones. A, B Representative fluorescent images of the cell migration assays performed with the OPBG-DIPG002 derived clones (A) 1C5 (Venus) and 2B4 (m-Orange2), and the OPBG-GBM002 clones (B) 5E2 (Venus) and 1D3 (m-Orange2), in mono-culture and in co-culture (overlay of m-Orange2 and Venus) and in presence or absence of 10 μM and 20 μM of GW4869 or DMSO as vehicle control for 48 h. The graph bar shows the quantification of the cell migration areas after segmentation and analysis of the images using the Harmony software. Images were acquired with the Operetta CLS every 30 min over 48 h. At the 48 h time point, Scale bar = 200 μm. Data are mean ± SD, n = 2 with 6 different technical repeats. (****) p < 0.0001; (***) p < 0.001; (**) p < 0.01; (*) p < 0.05