Fig. 2

Single-cell-derived clones display different genomic and transcriptomic features. A, B Next-generation sequencing was carried out on the OPBG-DIPG002 (A), OPBG-GBM002 (B) cell lines and the respective tumour samples and corresponding derived clones. Blood samples were used as a control. The heatmaps show the gene variants identified in at least one specimen. C, D Heatmaps showing the differential gene expression obtained from RNA sequencing analysis performed with cells from OPBG-DIPG002 (C), OPBG-GBM002 (D) and from the corresponding derived clones. Gene set enrichment analysis was performed for the top 50 most highly differentially expressed genes between the OPBG-DIPG002 derived clones 2B4 and 1C5 (C) and the OPBG-GBM002 derived clones 5E2 and 1D3 (D). All cell preparations were sequenced n = 1 and statistical comparisons were made by Gene Set Enrichment Analysis