Fig. 1

Localization of PrP and α/ꞵ synuclein in TIA1-positive stress granules after oxidative stress induction in HeLa cells. A-D Representative immunofluorescence results. Localization of endogenous TIA1, PrP, and α/β synuclein was investigated in sodium-arsenite-treated {(0.6 mM; 60 min) (stress)} and untreated (control) HeLa cells using co-immunofluorescence and confocal microscopy. Counter-staining was performed to visualize cell nuclei, scale bar = 50 μm. White arrows show yellow cytoplasmic dots with TIA1 and PrP co-localization in granules. Three different antibodies were used against prion protein (SAF 32, SAF 70, and 12F10). E and F The granule formation propensity of PrP, and synuclein was assessed by the catGRANULE algorithm. G-J Quantification of the number of TIA1-positive stress granules