Fig. 6
Mechanisms of RRM2-Mediated AKT Activation in Prostate Cancer. A RRM 2 knockdown somewhat reduced the phosphorylation level of AKT while improving the antitumor effect of docetaxel. B, C Immunoprecipitation, silver staining and mass spectrometry analysis shows that ANXA1 is a binding partner of RRM2 in PCa,and the Log2 ratio indicates the abundant presence of ANXA1 in the RRM2 immunoprecipitates. D Protein interaction network (PPI) analysis indicating the interaction relationship between RRM2 and ANXA1. E Coimmunoprecipitation (Co-IP) assay results confirming the interaction between RRM2 and ANXA1 in PC3 and DU145 cells. F ANXA1 silencing decreased the phosphorylation of AKT in both cell classes. G The expression level and colocalization of RRM2 and ANXA1 in PC3 and DU145 cells