Fig. 4

Functional analysis of RRM2 in prostate cancer progression. A, B Analysis of RRM2 mRNA and protein levels across different cell lines revealed heightened expression in PC3 and DU145 cells in comparison to RWPE-1 cell lines. C Effective downregulation of RRM2 in PC3 and DU145 cells, which originally exhibited elevated RRM2 expression, was achieved through the transfection of two independent siRNAs. D–H Notably diminished cloning and proliferation capacity of prostate cancer cells was observed consequent to RRM2 knockdown. I–L A significant increase in the rates of necroptosis and apoptosis observed in prostate cancer cells following RRM2 knockdown