Table 2 Primers used in this research for qRT-PCR
From: Cas13b-mediated RNA targeted therapy alleviates genetic dilated cardiomyopathy in mice
Gene | Forword primer (5’ − 3’) | Reverse primer (5’ − 3’) |
|---|---|---|
hTNNT2 | CTATCTCCTCCAGATGGCCC | ACTCTCTCTCCATCGGGGAT |
Tnnt2 | AGAGGACACCAAACCCAAGC | CTCCCGCTCATTGCGAAT |
Nppb | GTCAGTCGTTTGGGCTGTA | GCTATGTTTATTATGTTGTGGC |
Nppa | TCCGATAGATCTGCCCTCTT | CTCCAATCCTGTCAATCCTACC |
Col3a1 | ACTTGGAATTGCAGGGCTAAC | CTGGCTCCTGGTTTTCCACT |
Postn | TGACATACGCAGAGGACTG | TCAATGACATGGACGACAC |
ACTIN | GTGACAGCAGTCGGTTGG | GCAATGCTATCACCTCCC |
Actin | AGAGGACACCAAACCCAAGC | CTCCCGCTCATTGCGAAT |