Table 1 The sequences of sgRNAs designed for the targeted mutation site
From: Cas13b-mediated RNA targeted therapy alleviates genetic dilated cardiomyopathy in mice
sgRNA Number | sgRNA Sequence (5’ − 3’) |
|---|---|
sgRNA1 | GCTCCTTCTCCCGCTCATTCCAGATGCGCT |
sgRNA2 | TCCTTCTCCCGCTCATTCCAGATGCGCTGC |
sgRNA3 | CTTCTCCCGCTCATTCCAGATGCGCTGCTG |
sgRNA4 | TCTCCCGCTCATTCCAGATGCGCTGCTGCT |
sgRNA5 | TCCCGCTCATTCCAGATGCGCTGCTGCTCG |
sgRNA6 | CCGCTCATTCCAGATGCGCTGCTGCTCGGC |
sgRNA7 | GCTCATTCCAGATGCGCTGCTGCTCGGCCC |
sgRNA8 | TCATTCCAGATGCGCTGCTGCTCGGCCCGC |
sgRNA9 | TCCAGATGCGCTGCTGCTCGGCCCGCTCTG |
sgRNA10 | CCGCTCCTTCTCCCGCTCATTCCAGATGCG |