Fig. 3
From: Cas13b-mediated RNA targeted therapy alleviates genetic dilated cardiomyopathy in mice
Effective knockdown of hTNNT2R141W mRNA expression through PspCas13b in vivo. (A) Schematic of the experimental procedure. (B) Quantification of hTNNT2 mRNA levels in hearts from AAV9-PspCas13b-sgTNNT2-injected (n = 6) and noninjected R141W mice (n = 3). (C) Quantification of the relative expression of the mouse Tnnt2 gene in DCM and DCM + AAV9 cardiomyocytes by qRT-PCR using the 2-∆∆Ct method (n = 3). The bar graphs show the mean ± SEM. NS is not significant; *p < 0.05 according to Student’s t test