Fig. 2
From: c-Jun as a one-way valve at the naive to primed interface
c-Jun N-terminal Kinase inhibitor facilitates primed to naïve Transition. A. Work model shows JNK inhibitor (SP600125) in primed to naive transition (PNT). B, Flow cytometry analysis represent reprogramming efficiency increased with a higher dose of JNKi. C, D. A dose-response graph for JNKi in promoting primed to naive transition. This experiment was performed with three different EpiSC cell lines called EpiSC B4 (this cell line was named EpiSC in the text, and used for this study), EpiSC B1, EpiSC B2. JNKi was used with 3 doses for 3 days during primed to naive transition (PNT) and scored for GFP positive colonies. Data are from 6 biological replicates in 2 independent experiments and shown as the mean ± SEM. *** indicates P value < 0.001, one-way ANOVA with Sidak correction between the DMSO and the dosing groups. E. Phase and GFP images of iPSC. Scale bar = 100 μm. F. Karyotypes of iPSC. G. Chimeric mice from iPSC.