Fig. 1From: Liver organoid culture methodsMainstream methods for generating PSC-derived liver organoids. Method I: Organoids are formed on the basis of PSC-induced mature hepatocytes; Method II: Spheroids are formed during the process of PSC differentiation, and immature spheroids are then further differentiated into mature hepatic organoids. PSCs pluripotent stem cells, DE definitive endoderm, IHs immature hepatocytes, MHs mature hepatocytes, Hos hepatic organoids, PDF posterior foregutBack to article page