Fig. 9

Spermatogonia loss induced by E4f1 inactivation is not dependent on P53. A Immunofluorescence staining for P53(S15) (red) and TRA98 (green) in sections of PD6 controls and E4f1 cKO testes. B Immunofluorescence staining for P53(S15) (red) and TRA98 in the testis of control, E4f1 cKO and E4f1;Trp53 dcKO testes. C Quantification of P53 (S15) per 500 germ cells in testis sections from PD6 controls and E4f1 cKO mice. D Immunofluorescence staining for LIN28A (red) and SOX9 (green) in sections of PD35 control, E4f1 cKO, and E4f1;Trp53 dKO testes. E Quantification of undifferentiated spermatogonia per 500 Sertoli cells in testis sections from PD35 controls, E4f1 cKO, and E4f1;Trp53 dKO mice. F Percentage of γH2AX-positive undifferentiated spermatogonia in testis sections from PD35 control, E4f1 cKO and E4f1;P53 dcKO testes. G Percentage of apoptotic undifferentiated spermatogonia in testis sections from PD35 control, E4f1 cKO and E4f1;Trp53 dcKO testes. H Percentage of EdU-positive undifferentiated spermatogonia in testis sections from PD35 control, E4f1 cKO and E4f1;P53 dcKO testes. I Immunofluorescence staining for TRA98 (green) and SOX9 (red) in sections of PD60 control, E4f1 cKO, and E4f1;P53 dKO testes. *P < 0.05, **P < 0.01, ***P < 0.001, Student’s t test. n = 3