Fig. 2From: Menin orchestrates hepatic glucose and fatty acid uptake via deploying the cellular translocation of SIRT1 and PPARγHigher Menin expression in hepatocytes suppressed fatty acid transport by interacting with PPARγ and targeting its downstream genes. A. Menin protein was over-expressed in hepatocytes at 24 h after transfection with pcDNA3.1-mMen1 (mMen1) or an empty vector (Ctrl). The representative Western blot images are shown in the inserted panel, where ex-Menin represents exogenously over-expressed Menin, and en-Menin represents endogenous Menin. B. Downstream target genes such as Fabp3, Fabp4, Fabp5, and Cd36 were found to be inhibited upon Menin over-expression. *P < 0.05, **P < 0.01, *** P < 0.001. C. Higher Menin expression in hepatocytes inhibited the protein expression of PPARγ, FABP3, FABP4, and FABP5. D. Menin was found to interact with PPARγ in plain mouse hepatocytes via a Menin-IP assayBack to article page