Fig. 5

p110α Y508F heterozygous mutation impairs AKT phosphorylation. A-C p110α Y508F mutation affects AKT phosphorylation. Parental cells and their corresponding p110α Y508F KI mutant cells were serum-starved overnight (A) and then treated with EGF or insulin for 15 min (B, C). Cell lysates were blotted with indicated antibodies. D-G AKT reconstitution rescues the proliferation and migration defects caused by p110α Y508F heterozygous mutation. Myristoylated-AKT1 (myr-AKT) was overexpressed in HCT116 p110α Y508F KI mutant cells. p-AKT and total AKT protein levels were evaluated by Western blots (D). Indicated cells were analyzed with for proliferation (E); migration (F); and colony formation (G). Two-tailed unpaired t test (F, G) and two-way ANOVA (E), *p < 0.05, **p < 0.01, ***p < 0.001, ns, not significant