Fig. 2

p110α Y317F mutation impairs the proliferation, migration, and invasion of CRC cells. A-D p110α Y317F mutation impaired the growth of CRC cells. Cell lines with indicated genotype were analyzed for cell proliferation (A), colony formation (B, C), and xenograft tumor growth (D). Y317 indicates parental cells; Y317F indicates p110α Y317F KI mutant cells. E-J p110α Y317F mutation impaired CRC metastasis. Cell lines with indicated genotype were analyzed for cell migration (E, F) and invasion (G, H) by transwell assays. The indicated cells were injected into nude mice through tail veins. Two months later, mice were sacrificed. The H&E staining images (I) and numbers of lung metastatic nodules (J) were present. Two-tailed unpaired t test (C, F, H, J) and two-way ANOVA (A, D), *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001