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Fig. 8 | Cell & Bioscience

Fig. 8

From: Down-regulated FTO and ALKBH5 co-operatively activates FOXO signaling through m6A methylation modification in HK2 mRNA mediated by IGF2BP2 to enhance glycolysis in colorectal cancer

Fig. 8

Silenced FTO/ALKBH5 promotes METTL3 while inhibits METTL14 to accelerating the development of CRC A The protein levels of METTL3, METTL14, and WTAP, when FTO and ALKBH5 were knockdown in HCT116 cells, showed by western blot. B Western blot indicated the expression of FTO and ALKBH5 in METTL3/METTL14/WTAP deficient cells. C The efficiency of METTL3 knockdown in CRC cells was verified by western blot. D Viability of METTL3 knockdown cells detected by CCK-8 assay E, F EdU analysis of the effects of METTL3 knockdown (magnification times, 200 ×). G, H Representative images of colony formation assay and colony number analysis. I, J Cell migration and invasion assays of CRC cells treated with METTL3 knockdown (magnification times, 100 ×). K The expression of METTL14 in METTL14 knockdown cells is indicated by western blot. L–P The results of CCK-8, colony formation, and EdU (magnification times, 200 ×) assays in METTL14 knockdown cells which be used to elevate cell proliferation. Q, R Migration and invasion were detected by transwell with METTL14 knockdown in CRC cells (magnification times, 100 ×). **p < 0.01; ***p < 0.001

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Cell & Bioscience

ISSN: 2045-3701

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