Fig. 7

Plasma exosomes isolated from CrebH^−/− mice with IBD stimulate hepatic inflammation in the liver of WT mice treated with DSS. A Schematic representation of the experimental schedule. Plasma exosomes from WT and CrebH^−/− mice after administration of DSS for 7 days were isolated and then WT mice were treated with isolated exosomes (200 ug protein concentration) at 0 and 4 days of DSS schedule. PBS were used as a control for exosome treatment. B AST and ALT levels. C Gene expression of MAdCAM-1, ICAM1, VCAM1, and VAP in the livers of each group. D Immunohistochemistry for MAdCAM-1 and CD3 (upper), confirmed by mRNA expression of CD3, CD4, or CD8 (lower). The scale bars represent 200 μm (MAdCAM-1) and 100 μm (CD3), respectively. E Gene expression of IL-1β, IL-6, TNFα, and IL-10 in the livers of each group. F Gene expression of Acot3, Cyp4a10, Cyp4a14, Fabp4, and Mcam in the livers of each group. G Gene expression of Adamts, Anxa2, Ctss, Dcn, Itgb3, and Lgals1 in the livers of each group. *P < 0.05, **P < 0.01, ***P < 0.001. Statistical analysis was performed using a two-tailed Student’s t-test. Error bars represent the mean ± SEM