Fig. 6

Exosomal miRNA from plasma of WT and CrebH^−/− mice and the effect of miR-29a-3p on expression of MAdCAM-1 and VAP1. A Venn diagram of exosomal miRNA altered between WT-control vs. WT-DSS or WT-DSS vs. CrebH^−/−-DSS. B Heatmaps showing hierarchical clustering of differentially expressed exosomal miRNA between DSS vs. control (left) and CrebH^−/−-DSS vs. WT-DSS (right). C miR-29a-3p levels analyzed by miRNA profiling of stable HepG2 cells overexpressing pcDNA3 or CrebH. D Exosomal miR-29a-3p concentration was validated by qRT-PCR in exosomes released from HepG2 cells overexpressing pcDNA3.1 or CrebH. E miRNA profiling data for exosomal miR-29a levels of CrebH^−/−-DSS compared with WT-DSS. F Effect of the mimic on miR-29a-3p expression in bEnd.3 cells. G, H Gene expression of MAdCAM-1 and VAP1 response to miR-29a mimic. I Target sequence on AKT 3′ UTR against miR-29a-3p (upper) and representative western blot data for AKT1, AKT2, and TNF-R1 (lower). J, TNF-R1 mRNA levels of each groups. *P < 0.05, **P < 0.01, or ***P < 0.001. Statistical analysis was performed using a two-tailed Student’s t-test. Representative data are from at least two independent experiments. Error bars represent the mean ± SEM