Fig. 3

CrebH deficiency leads to increased T lymphocyte infiltration via enhanced adhesion molecules in the liver. A Cd3⁺Cd4⁺ and Cd3⁺Cd8⁺ double-positive cells were determined by flow cytometric analysis of cell population in the liver of WT (n = 6) and CrebH^−/− (n = 6) mice after DSS treatment. *P < 0.05. B, C mRNA levels of MAdCAM-1 and VAP1 in the liver from WT and CrebH^−/− mice were measured by qRT-PCR. **P < 0.01, or ***P < 0.001. D Protein levels of MAdCAM-1 in the liver from WT and CrebH^−/− mice were evaluated by immunoblotting using specific antibodies against each protein, and their optical intensities were normalized by β-actin. *P < 0.05. E Representative immunohistochemistry images on MAdCAM-1 in the liver from WT and CrebH^−/− mice. The scale bar represents 200 μm. F Gene expression of MAdCAM-1, VAP1, and ICAM1 was estimated in the colon (left) and ileum (right) of WT (n = 5) and CrebH^−/− (n = 6) mice treated with DNBS for 2 days. *P < 0.05, **P < 0.01. Statistical analysis was performed using a two-tailed Student’s t-test. Error bars represent the mean ± SEM